Its entrance is next to the provincial road of Sitia-Karydi, between the villages of Sitanos and Katsidoni in an altitude of 684 meters. The main entrance (there are two) has a diameter of 2 meters and has an intense downwards inclination, which exlpains its name ("Latsidi" means "hole" in Eastern Crete). Right after the entrance, a large chamber is revealed, again with a downwards inclination. Its ground is covered with stones of many sizes, clay, and other material, such as wood, branches and leaves.

After passing this chamber, and for the next 250 meters, there are five large, uneven chambers, which are connected with small passages. After passing the sixth chamber there are small tunnels that periodically become narrow. Water flows in their pavement, which increases during the rain seasons. It is believed that the cave of Okso Latsidi used to be part of an underground river.